| 1 | |  Stevenage FC | 44 | 30 | 9 | 5 | 79 | 24 | 55 | 99 | G17:00 | 05/04/10 | R 43  Wimbledon 0 Stevenage FC 3 G17:00 | 10/04/10 | R 44 Stevenage FC 2 Forest Green Rovers 0 G21:45 | 14/04/10 | R 32 Altrincham 0 Stevenage FC 1 G17:00 | 17/04/10 | R 45 Kidderminster Harriers FC 0 Stevenage FC 2 G21:45 | 20/04/10 | R 40 Gateshead 0 Stevenage FC 1 G17:00 | 24/04/10 | R 46 Stevenage FC 1 York City FC 0 |
| 2 | |  Luton Town | 44 | 26 | 10 | 8 | 84 | 40 | 44 | 88 | B17:00 | 10/04/10 | R 44 Tamworth 1 Luton Town 1 G21:45 | 13/04/10 | R 26 Luton Town 6 Histon FC 3 B17:00 | 17/04/10 | R 45 Luton Town 0 Altrincham 0 B17:00 | 24/04/10 | R 46 AFC Rushden And Diamonds 1 Luton Town 1 M22:00 | 29/04/10 | R semifinal York City FC 1 Luton Town 0 M17:00 | 03/05/10 | R semifinal Luton Town 0 York City FC 1 |
| 3 | |  Oxford United | 44 | 25 | 11 | 8 | 64 | 31 | 33 | 86 | G17:00 | 17/04/10 | R 45 Oxford United 2 Mansfield Town 0 G21:45 | 20/04/10 | R 32 Oxford United 1 Wrexham 0 M17:00 | 24/04/10 | R 46 Eastbourne Borough 1 Oxford United 0 B21:45 | 29/04/10 | R semifinal AFC Rushden And Diamonds 1 Oxford United 1 G17:00 | 03/05/10 | R semifinal Oxford United 2 AFC Rushden And Diamonds 0 G19:00 | 16/05/10 | R final Oxford United 3 York City FC 1 |
| 4 | |  AFC Rushden And Diamonds | 44 | 22 | 13 | 9 | 77 | 39 | 38 | 79 | G17:00 | 10/04/10 | R 44 AFC Rushden And Diamonds 2 Ebbsfleet United FC 0 M21:45 | 13/04/10 | R 36 Mansfield Town 3 AFC Rushden And Diamonds 2 G17:00 | 17/04/10 | R 45 Wrexham 0 AFC Rushden And Diamonds 1 B17:00 | 24/04/10 | R 46 AFC Rushden And Diamonds 1 Luton Town 1 B21:45 | 29/04/10 | R semifinal AFC Rushden And Diamonds 1 Oxford United 1 M17:00 | 03/05/10 | R semifinal Oxford United 2 AFC Rushden And Diamonds 0 |
| 5 | |  York City FC | 44 | 22 | 12 | 10 | 62 | 35 | 27 | 78 | G21:45 | 13/04/10 | R 34 York City FC 3 Barrow 0 B17:00 | 17/04/10 | R 45 York City FC 1 Gray Athletics 1 M17:00 | 24/04/10 | R 46 Stevenage FC 1 York City FC 0 G22:00 | 29/04/10 | R semifinal York City FC 1 Luton Town 0 G17:00 | 03/05/10 | R semifinal Luton Town 0 York City FC 1 M19:00 | 16/05/10 | R final Oxford United 3 York City FC 1 |
| 6 | |  Kettering Forest | 44 | 18 | 12 | 14 | 51 | 41 | 10 | 66 | M21:45 | 30/03/10 | R 29 Kettering Forest 0 AFC Rushden And Diamonds 3 B17:00 | 03/04/10 | R 42 Gray Athletics 0 Kettering Forest 0 B17:00 | 05/04/10 | R 43 Kettering Forest 1 Histon FC 1 G17:00 | 17/04/10 | R 45 Kettering Forest 4 Eastbourne Borough 0 B21:45 | 20/04/10 | R 32 Kettering Forest 1 Crawley Town 1 M17:00 | 24/04/10 | R 46 Salisbury FC 2 Kettering Forest 0 |
| 7 | |  Crawley Town | 44 | 19 | 9 | 16 | 50 | 57 | -7 | 66 | G17:00 | 05/04/10 | R 43 Crawley Town 1 Heyes & Yeading 0 G17:00 | 10/04/10 | R 44 Histon FC 0 Crawley Town 1 M21:45 | 13/04/10 | R 30 Forest Green Rovers 1 Crawley Town 0 M21:45 | 15/04/10 | R 40 Barrow 4 Crawley Town 1 G17:00 | 17/04/10 | R 45 Crawley Town 2 Tamworth 0 B21:45 | 20/04/10 | R 32 Kettering Forest 1 Crawley Town 1 |
| 8 | |  Wimbledon | 44 | 18 | 10 | 16 | 61 | 47 | 14 | 64 | M17:00 | 05/04/10 | R 43 Wimbledon 0 Stevenage FC 3 M21:45 | 07/04/10 | R 40 York City FC 5 Wimbledon 0 G17:00 | 10/04/10 | R 44 Mansfield Town 0 Wimbledon 1 M21:45 | 13/04/10 | R 30 Wimbledon 0 Gray Athletics 2 M21:45 | 20/04/10 | R 36 Wimbledon 0 Tamworth 1 M17:00 | 24/04/10 | R 46 Gateshead 1 Wimbledon 0 |
| 9 | |  Mansfield Town | 44 | 17 | 11 | 16 | 69 | 60 | 9 | 62 | M17:00 | 03/04/10 | R 42 Mansfield Town 0 Wrexham 1 M17:00 | 05/04/10 | R 43 Cambridge United 3 Mansfield Town 2 M17:00 | 10/04/10 | R 44 Mansfield Town 0 Wimbledon 1 G21:45 | 13/04/10 | R 36 Mansfield Town 3 AFC Rushden And Diamonds 2 M17:00 | 17/04/10 | R 45 Oxford United 2 Mansfield Town 0 B17:00 | 24/04/10 | R 46 Mansfield Town 3 Kidderminster Harriers FC 3 |
| 10 | |  Cambridge United | 44 | 15 | 14 | 15 | 65 | 53 | 12 | 59 | M17:00 | 03/04/10 | R 42 Gateshead 2 Cambridge United 0 G17:00 | 05/04/10 | R 43 Cambridge United 3 Mansfield Town 2 M17:00 | 10/04/10 | R 44 Salisbury FC 2 Cambridge United 1 B21:45 | 13/04/10 | R 22 Oxford United 0 Cambridge United 0 G17:00 | 17/04/10 | R 45 Cambridge United 4 Heyes & Yeading 1 G17:00 | 24/04/10 | R 46 Altrincham 0 Cambridge United 2 |
| 11 | |  Wrexham | 44 | 15 | 13 | 16 | 45 | 39 | 6 | 58 | G17:00 | 03/04/10 | R 42 Mansfield Town 0 Wrexham 1 B17:00 | 05/04/10 | R 43 Wrexham 0 Gateshead 0 G17:00 | 10/04/10 | R 44 Gray Athletics 0 Wrexham 2 M17:00 | 17/04/10 | R 45 Wrexham 0 AFC Rushden And Diamonds 1 M21:45 | 20/04/10 | R 32 Oxford United 1 Wrexham 0 G17:00 | 24/04/10 | R 46 Heyes & Yeading 0 Wrexham 1 |
| 12 | |  Salisbury FC | 44 | 21 | 5 | 18 | 58 | 63 | -5 | 58 | M17:00 | 05/04/10 | R 43 Oxford United 1 Salisbury FC 0 G17:00 | 10/04/10 | R 44 Salisbury FC 2 Cambridge United 1 G21:45 | 13/04/10 | R 36 Salisbury FC 3 Heyes & Yeading 1 G17:00 | 17/04/10 | R 45 Barrow 0 Salisbury FC 1 G21:45 | 20/04/10 | R 39 Salisbury FC 4 Altrincham 1 G17:00 | 24/04/10 | R 46 Salisbury FC 2 Kettering Forest 0 |
| 13 | |  Kidderminster Harriers FC | 44 | 15 | 12 | 17 | 57 | 52 | 5 | 57 | M21:45 | 08/04/10 | R 36 Barrow 1 Kidderminster Harriers FC 0 G17:00 | 10/04/10 | R 44 Gateshead 0 Kidderminster Harriers FC 2 B21:45 | 15/04/10 | R 25 Heyes & Yeading 2 Kidderminster Harriers FC 2 M17:00 | 17/04/10 | R 45 Kidderminster Harriers FC 0 Stevenage FC 2 M21:45 | 20/04/10 | R 22 Kidderminster Harriers FC 1 Barrow 2 B17:00 | 24/04/10 | R 46 Mansfield Town 3 Kidderminster Harriers FC 3 |
| 14 | |  Altrincham | 44 | 13 | 15 | 16 | 53 | 51 | 2 | 54 | M17:00 | 05/04/10 | R 43 York City FC 2 Altrincham 1 M17:00 | 10/04/10 | R 44 Altrincham 0 Oxford United 1 M21:45 | 14/04/10 | R 32 Altrincham 0 Stevenage FC 1 B17:00 | 17/04/10 | R 45 Luton Town 0 Altrincham 0 M21:45 | 20/04/10 | R 39 Salisbury FC 4 Altrincham 1 M17:00 | 24/04/10 | R 46 Altrincham 0 Cambridge United 2 |
| 15 | |  Barrow | 44 | 13 | 13 | 18 | 50 | 67 | -17 | 52 | B17:00 | 10/04/10 | R 44 Heyes & Yeading 1 Barrow 1 M21:45 | 13/04/10 | R 34 York City FC 3 Barrow 0 G21:45 | 15/04/10 | R 40 Barrow 4 Crawley Town 1 M17:00 | 17/04/10 | R 45 Barrow 0 Salisbury FC 1 G21:45 | 20/04/10 | R 22 Kidderminster Harriers FC 1 Barrow 2 B17:00 | 24/04/10 | R 46 Histon FC 2 Barrow 2 |
| 16 | |  Tamworth | 44 | 11 | 16 | 17 | 42 | 52 | -10 | 49 | G17:00 | 03/04/10 | R 42 Tamworth 3 Barrow 0 G17:00 | 05/04/10 | R 43 Forest Green Rovers 3 Tamworth 4 B17:00 | 10/04/10 | R 44 Tamworth 1 Luton Town 1 M17:00 | 17/04/10 | R 45 Crawley Town 2 Tamworth 0 G21:45 | 20/04/10 | R 36 Wimbledon 0 Tamworth 1 M17:00 | 24/04/10 | R 46 Tamworth 3 Ebbsfleet United FC 4 |
| 17 | |  Heyes & Yeading | 44 | 12 | 12 | 20 | 59 | 85 | -26 | 48 | M17:00 | 05/04/10 | R 43 Crawley Town 1 Heyes & Yeading 0 B17:00 | 10/04/10 | R 44 Heyes & Yeading 1 Barrow 1 M21:45 | 13/04/10 | R 36 Salisbury FC 3 Heyes & Yeading 1 B21:45 | 15/04/10 | R 25 Heyes & Yeading 2 Kidderminster Harriers FC 2 M17:00 | 17/04/10 | R 45 Cambridge United 4 Heyes & Yeading 1 M17:00 | 24/04/10 | R 46 Heyes & Yeading 0 Wrexham 1 |
| 18 | |  Histon FC | 44 | 11 | 13 | 20 | 44 | 67 | -23 | 46 | M17:00 | 03/04/10 | R 42 Histon FC 1 Wimbledon 3 B17:00 | 05/04/10 | R 43 Kettering Forest 1 Histon FC 1 M17:00 | 10/04/10 | R 44 Histon FC 0 Crawley Town 1 M21:45 | 13/04/10 | R 26 Luton Town 6 Histon FC 3 M17:00 | 17/04/10 | R 45 Forest Green Rovers 2 Histon FC 0 B17:00 | 24/04/10 | R 46 Histon FC 2 Barrow 2 |
| 19 | |  Eastbourne Borough | 44 | 11 | 13 | 20 | 42 | 72 | -30 | 46 | G21:45 | 30/03/10 | R 32 Eastbourne Borough 2 Gateshead 1 G17:00 | 03/04/10 | R 42 Eastbourne Borough 1 Forest Green Rovers 0 M17:00 | 05/04/10 | R 43 Ebbsfleet United FC 3 Eastbourne Borough 2 G17:00 | 10/04/10 | R 44 Eastbourne Borough 3 York City FC 1 M17:00 | 17/04/10 | R 45 Kettering Forest 4 Eastbourne Borough 0 G17:00 | 24/04/10 | R 46 Eastbourne Borough 1 Oxford United 0 |
| 20 | |  Gateshead | 44 | 13 | 7 | 24 | 46 | 69 | -23 | 45 | G17:00 | 03/04/10 | R 42 Gateshead 2 Cambridge United 0 B17:00 | 05/04/10 | R 43 Wrexham 0 Gateshead 0 M17:00 | 10/04/10 | R 44 Gateshead 0 Kidderminster Harriers FC 2 M17:00 | 17/04/10 | R 45 Ebbsfleet United FC 2 Gateshead 0 M21:45 | 20/04/10 | R 40 Gateshead 0 Stevenage FC 1 G17:00 | 24/04/10 | R 46 Gateshead 1 Wimbledon 0 |
| 21 | |  Forest Green Rovers | 44 | 12 | 9 | 23 | 50 | 76 | -26 | 45 | M17:00 | 03/04/10 | R 42 Eastbourne Borough 1 Forest Green Rovers 0 M17:00 | 05/04/10 | R 43 Forest Green Rovers 3 Tamworth 4 M17:00 | 10/04/10 | R 44 Stevenage FC 2 Forest Green Rovers 0 G21:45 | 13/04/10 | R 30 Forest Green Rovers 1 Crawley Town 0 G17:00 | 17/04/10 | R 45 Forest Green Rovers 2 Histon FC 0 M17:00 | 24/04/10 | R 46 Gray Athletics 2 Forest Green Rovers 1 |
| 22 | |  Ebbsfleet United FC | 44 | 12 | 8 | 24 | 50 | 82 | -32 | 44 | M17:00 | 20/03/10 | R 40 Ebbsfleet United FC 1 Luton Town 6 M17:00 | 02/04/10 | R 42 Salisbury FC 3 Ebbsfleet United FC 1 G17:00 | 05/04/10 | R 43 Ebbsfleet United FC 3 Eastbourne Borough 2 M17:00 | 10/04/10 | R 44 AFC Rushden And Diamonds 2 Ebbsfleet United FC 0 G17:00 | 17/04/10 | R 45 Ebbsfleet United FC 2 Gateshead 0 G17:00 | 24/04/10 | R 46 Tamworth 3 Ebbsfleet United FC 4 |
| 23 | |  Gray Athletics | 44 | 5 | 13 | 26 | 35 | 91 | -56 | 26 | B17:00 | 03/04/10 | R 42 Gray Athletics 0 Kettering Forest 0 M17:00 | 05/04/10 | R 43 Luton Town 6 Gray Athletics 0 M17:00 | 10/04/10 | R 44 Gray Athletics 0 Wrexham 2 G21:45 | 13/04/10 | R 30 Wimbledon 0 Gray Athletics 2 B17:00 | 17/04/10 | R 45 York City FC 1 Gray Athletics 1 G17:00 | 24/04/10 | R 46 Gray Athletics 2 Forest Green Rovers 1 |
